Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands
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منابع مشابه
Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands
Here we describe a novel crosslinker and its application as a biotin-transfer reagent to identify cell surface receptors of soluble protein ligands on live cells. This crosslinker contains three functional groups: an aldehyde-reactive aminooxy group, a sulfhydryl, and a biotin (ASB). It is readily synthesized via a 3-step addition reaction using standard solid-phase peptide synthesis methods an...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/srep46574